Acid-fast Stain

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What is Acid-fast Stain?

The acid-fast stain is a laboratory test that determines if a sample of tissue, blood, or other body substance is infected with the bacteria that causes tuberculosis and other illnesses.



Alternative names



How the Test is Performed

Your health care provider will collect a sample of blood, urine, stool, sputum, bone marrow, or tissue, depending on the location of the suspected infection. The sample is then sent to a laboratory, where a small amount is placed on a glass slide, stained, and heated. The cells in the sample hold onto the dye. The lab team member washes the slide with an acid solution and applies a different stain. The bacteria that hold onto the first dye are considered "acid-fast" because they resist the...



What is Acid-fast Stain?

The acid-fast stain is a laboratory test that determines if a sample of tissue, blood, or other body substance is infected with the bacteria that causes tuberculosis and other illnesses.

How the Test is Performed

Your health care provider will collect a sample of blood, urine, stool, sputum, bone marrow, or tissue, depending on the location of the suspected infection.

The sample is then sent to a laboratory, where a small amount is placed on a glass slide, stained, and heated. The cells in the sample hold onto the dye. The lab team member washes the slide with an acid solution and applies a different stain.

The bacteria that hold onto the first dye are considered "acid-fast" because they resist the acid wash. This type of bacteria is associated with tuberculosis and other infections.

How to Prepare for the Test

Preparation depends on how the sample is collected. Your health care provider will tell you how to prepare.

How the Test Will Feel

The amount of discomfort depends on how the sample is collected.

Why the Test is Performed

The test can tell if you are infected with mycobacteria, the organism that causes tuberculosis and related infections.

Normal Results

A normal result means no acid-fast bacteria were found on the stained sample.

Note: Normal value ranges may vary slightly among different laboratories. Talk to your doctor about the meaning of your specific test results.

What Abnormal Results Mean

Abnormal results may indicate an infection with an acid-fast bacteria. Acid-fast bacteria include those that cause tuberculosis, nontuberculous infections, and nocardia infections.

Risks

Risks depend on how the sample is collected. Ask your health provider to explain the risks and benefits of the medical procedure.

Content provided by:

A.D.A.M., Inc. is accredited by URAC, also known as the American Accreditation HealthCare Commission (www.urac.org). URAC's accreditation program is an independent audit to verify that A.D.A.M. follows rigorous standards of quality and accountability. A.D.A.M. is among the first to achieve this important distinction for online health information and services. Learn more about A.D.A.M.'s editorial policy, editorial process and privacy policy. A.D.A.M. is also a founding member of Hi-Ethics and subscribes to the principles of the Health on the Net Foundation (www.hon.ch). The information provided herein should not be used during any medical emergency or for the diagnosis or treatment of any medical condition. A licensed medical professional should be consulted for diagnosis and treatment of any and all medical conditions. Call 911 for all medical emergencies. Links to other sites are provided for information only -- they do not constitute endorsements of those other sites. © 1997- 2008 A.D.A.M., Inc. Any duplication or distribution of the information contained herein is strictly prohibited.

Review Date: .12/3/2007

Reviewed By: D. Scott Smith, M.D., MSc, DTM&H, Chief of Infectious Disease & Geographic Medicine, Kaiser Redwood City, CA & Adjunct Assistant Professor, Stanford University. Review provided by VeriMed Healthcare Network. Also reviewed by David Zieve, MD, MHA, Medical Director, A.D.A.M., Inc.12/3/2007

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