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How to Calculate Osmolarity of Glucose

author image Adam Cloe Ph.D./M.D.
Adam Cloe has been published in various scientific journals, including the "Journal of Biochemistry." He is currently a pathology resident at the University of Chicago. Cloe holds a Bachelor of Arts in biochemistry from Boston University, a M.D. from the University of Chicago and a Ph.D. in pathology from the University of Chicago.
How to Calculate Osmolarity of Glucose
Blood sample with high blood sugar result. Photo Credit: jarun011/iStock/Getty Images

Glucose is a sugar that is commonly found in the blood. The osmolarity of a glucose solution is a measurement of the concentration of glucose in a liquid. Glucose solutions are sometimes given to help bring blood glucose levels up in the event that a patient's blood glucose levels get too low. Calculating the osmolarity of a solution will allow you to understand how much glucose it contains, which can be helpful in predicting its effects on blood glucose levels.

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Step 1

Weigh the amount of glucose that will go into the solution. This is needed to calculate the number of "moles" of glucose, which is part of the equation for calculating the osmolarity of glucose, the University of Mississippi Medical Center explains.

Step 2

Divide the mass of glucose by 180. The molecular weight of glucose is 180 g per mole, says John Kimball, retired Harvard professor of immunology and biology and author of several textbooks. A mole is a unit of measurement that is used in calculating to osmolarity of a solution.

Step 3

Divide by the volume of the solution. This will give you the molarity of glucose; because glucose does not separate into two separate compounds when it dissolves, the osmolarity and the molarity of a glucose solution are the same, the University of Vermont explains.

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